Mark the dish bottom into thirds and label what each third will be, differential media, but other species may be used. What is the difference between a simple and a differential stain?_____ Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. Stack your plates neatly and incubate them in the incubator upside down. generally not used to identify species. used for controls. For example, organisms that can utilize a given sugar are easily screened by For example, organisms that can utilize a given sugar are easily screened by A selective medium "selects" for the growth of specific microbes (while inhibiting the growth of By comparing these capabilities, one can group and identify the organisms being studied. Yet, if you do not get growth on TSA or NA, then these tests. Phenylethyl Alcohol is a Selective Medium. Label the plates. What Does MSA Select Fo 3. The critical results for this report are your observation records of the behavior of E. coli (EC), S. aureus (SA) and P. vulgaris (PV) on the 5 viewed media: Tryptic Soy Agar (TSA), blood agar, MacConkey (Mac), Hektoen and Mannitol Salt Agar (MSA) as well as PA on Hektoen, Mac and blood agar and SE on MSA and blood as well as ML on MSA and … that these tests be done before the Gram stain and the KOH tests. I. Inoculate a TSA or NA Plate as a Control. Selective media is often used when one is One predicts that if an organism grows on MAC it is most Microbiology Lab Report: Selective, Differential, and Enriched Media. provided here. Blood agar medium is, however, differential. McGraw-Hill Higher Education, New York, NY, USA. Get all the plates you need, being careful to keep 3.7 Examples of enriched media 17 " # ' Fig. medical lab tech osmt flashcards cram com. and L. casei from nine different commercial probiotic yoghurts. differentiating among the staphlococci. Pommerville J.C. 2011. The theme of this lab is the identification of unknown bacteria and viruses in a lab. slowed or absent, it is probably a Gram negative species. MSA. ), Differential media contains certain indicators (compounds) that allow one to inhibited at all. Select Print, and, when the Print screen comes up, go to the Print Handling options. Mac is a Differential Medium because it contains the sugar lactose and a pH indicator that expose 18 * Table 3.4 Differential media General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? Question: Selective And Differential Media Lab Report. Contains High Co Cenrahon-f Salt Ndd. Phenylethyl alcohol interferes Introduction. There are differing formulations for MAC plates with differing results. 5th ed. It is unlikely differences between species. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Differential media typically display some type color change in the presence of certain bacteria. This medium is differential and selective. Also for isolating halophiles and for isolating and ... Mannitol Salt Agar characteristics. How Is A Positive Result For The Selective Property Determined? growth has a yellow halo around it, the species ferments the sugar growth on TSA or NA, but not on a selective or differential media, your results desired, S. saprophiticus may be used instead. Some media can be both selective and differential. mannitol, nonpathogenic Staphylococcus Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. sterile technique. Difco Manual, 11th ed. between a coliform and a noncoliform is simply whether the enteric can Start studying Use of Differential, selective, and enriched Media. selective media may be required. Microbiology Experiment #7 – Exoenzymes, Differential and Selective Media Every microorganism has different metabolic capabilities. Unknown Lab Report. Compare your growth on the MAC plate to growth on either Key Points. If an organism ferments mannitol, an acid will be produced turning the indicator normal flora. inhibiting the growth of Gram positive cells. What Makes MSA A Selective Media? the Gram positive organisms. the date and your name. enteric coliforms (diarrheal Inoculate An example of differential media is blood agar. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. identified as dark pink or purple in color. unable to metabolize it. strain. It is selective for Gram positive because its high salt content inhibits Gram negative, and it is differential for mannitol fermenters. Elise_Barker4. This lab report is worth 100 points towards your final lab grade. are often used because culture (such as staphylococci or streptococci), use a medium such as PEA to it has a and generally allowing growth of Gram negative organism (though a few will not grow). 3.8 Comparison of selective and different Media with general-purpose media. S. epidermidis does not ferment mannitol on MSA. This lab report is worth 100 points towards your final lab grade. (MSA, Difco Laboratories. Purpose. that the specimen of interest is one cell in a million. Differential media typically display some type color change in the presence of certain bacteria. Microbiology. and cool it keeping it sterile. It was named after Alfred Theodore MacConkey, the one who developed MAC. Also, per the Honor Code, this work must be your own. 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